A porcine enterovirus G associated with enteric disease contains a novel papain-like cysteine protease.

摘要: Identification of unknown pathogens in pigs displaying enteric illness is difficult due to the large diversity bacterial and viral species found within faecal samples. Current methods often require or isolation, testing only a limited number known using quantitative PCR analysis. Herein, faeces from two 25-day-old piglets with diarrhoea Texas, USA, were analysed by metagenomic next-generation sequencing rapidly identify possible pathogens. Our analysis included bioinformatics pipeline rapid short-read classification de novo genome assembly which resulted identification porcine enterovirus G (EV-G), complete substantial nucleotide differences (>30 %) among current sequences, novel non-structural protein similar sequence Torovirus papain-like cysteine protease (PLpro). This discovery led circulation an EV-G PLpro USA that has not been previously reported.