Emergence of vanA Enterococcus faecium in Denmark, 2005-15

摘要: Objectives To describe the changing epidemiology of vancomycin-resistant Enterococcus faecium and faecalis in clinical samples Denmark 2005-15 according to species van type, and, furthermore, investigate genetic relatedness E. isolates from 2015. Methods During 2005-14, all VRE were tested for presence vanA/B/C genes by PCR. In 2015, whole-genome sequenced. From WGS data, MLST STs extracted silico . Core-genome (cgMLST) analysis was performed isolates. Results 2005-15, 1043 vanA , 25 vanB 4 28 detected. The number 200 isolates/year 2013-15. 368 1 detected along with cgMLST subdivided into 33 cluster types (CTs), whereas isolate belonged a different CT. ST203-CT859 most prevalent (51%), followed ST80-CT14 (22%), ST117-CT24 (6%), ST80-CT866 (4%) ST80-CT860 (2%). Comparison cgMLST.org database, previous studies personal communications neighbouring countries revealed that novel emerged December 2014 spread south Sweden Faroe Islands during Conclusions increased due emergence several clones.