Loss of Adenoviral Receptor Expression in Human Bladder Cancer Cells: A Potential Impact on the Efficacy of Gene Therapy

摘要: There is great interest in the development of gene therapeutic strategies for treatment benign and malignant diseases. Recombinant adenovirus has a wide spectrum tissue specificity an efficient vector delivery system. Successful delivery, however, requires viral entry into target cells via specific receptor-mediated uptake. Recently, cDNA clone (the coxsackie receptor [CAR]) encoding 46-kDa protein was identified as group C (e.g., type 2 5). Currently, little known regarding expression adenoviral normal cancer. In this paper, we have documented significant difference levels that may be due to transcriptional regulation CAR several human bladder cancer cell lines. The differences these correlated with their sensitivity infection. Transfection receptor-negative line led increased virus binding susceptibility adenovirus-mediated delivery. Our results demonstrate variable among cells. This variability impact on outcome adenovirus-based therapy.