Comparison of flow cytometry and image morphometry in the quantitative analysis of cell population markers in the lymph node of sheep

作者: Gjermund Gunnes , Mette Valheim , Charles McL. Press , Aage Tverdal , Anne Storset

DOI: 10.1016/S0165-2427(03)00080-1

关键词:

摘要: Two approaches to the quantitative analysis of cell population markers in tissues are flow cytometry and image morphometry. To compare these methods, sheep lymph nodes were collected analysed for CD8+ CD21+ populations, which selected represent dispersed concentrated respectively. These two populations measured as a percentage total count (flow) or tissue area (morphometry). The also respective base (CD2+ cells CD8 MHC II+ CD21). A simple linear regression showed that when assessed area, measurements obtained with morphometry only correlated significantly not highly population. However, their population, significant correlation both populations. This study demonstrates node methods comparable, but distribution should be considered, unit measurement is chosen.

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