Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids allows access to masked epitopes in live neurons
作者： Perrais D , Choquet D , Choquet D , Doose S , Sauer M
DOI: 10.1101/2021.02.27.433189
关键词:
摘要: ABSTRACT Progress in biological imaging is intrinsically linked to advances labeling methods. The explosion the development of high-resolution and super-resolution calls for new approaches label targets with small probes. These should allow faithfully report localization target within resolution – typically nowadays a few nanometers - access any epitope target, native cellular tissue environment. We here complete pipeline using genetic code expansion non-canonical amino acids primary neurons that allows fluorescently masked epitopes transmembrane proteins live neurons, both dissociated culture organotypic brain slices. This allowed us image differential two glutamate receptor auxiliary complex their partner variety methods including widefield, confocal, dSTORM microscopy.
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