Treatment with low-dose cytokines reduces oxidative-mediated injury in perilesional keratinocytes from vitiligo skin

摘要: Abstract Background Vitiligo is a systemic dermatological disorder characterized by the loss of skin pigmentation due to melanocyte injury or aberrant functioning. Recent data underline its multifactorial etiology with significant involvement autoimmune and redox alterations. The major role in vitiligo cellular immunity displayed augmented Th1 Th17 suppressed TREGs Th2 lymphocyte populations. Our previous studies indicate marked imbalance perilesional ("PL", i.e. obtained from visibly unaffected surrounding depigmented area patients) keratinocytes where massive infiltration inflammatory cells takes place. No defined therapy exists for vitiligo. Although number approaches have been used induction cells, they may be associated off-target effects. Objective In order identify targeted approach treatment we, first, aimed investigate possible source ROS overproduction PL keratinocytes. Second, we tested effect low-dose selected cytokines, on intra- extracellular production, cell viability cycle Methods vitro study was conducted primary patients our studies. activity NADPH oxidase measured intact control keratinocytes, treated not luminometric assay. following cytokines were treatment: IL-10 IL-4 (produced Th2, respectively), basic fibroblasts growth factor (bFGF) neuropeptide β-endorphin (modulating resistance oxidative stress immune response, respectively). All at concentration 10fg/ml prepared sequential-kinetic-activation (SKA). Intracellular production analyzed flow cytometry using H2DCFDA propidium iodide dyes, respectively. Cell fluorometric resazurin reduction method. Results results suggest that represents one main sources Further, SKA IL-10, and, particularly, bFGF display positive dyshomeostasis experimental conditions, don't affect Conclusion preliminary IL-4, can proposed as new therapeutic tool treatment.