Comprehensive proteomics and functional annotation of mouse brown adipose tissue.
作者： Jing Li , Juan Li , Wei-Gang Zhao , Hai-Dan Sun , Zheng-Guang Guo
DOI: 10.1371/JOURNAL.PONE.0232084
关键词:
摘要: Knowledge about the mouse brown adipose tissue (BAT) proteome can provide a deeper understanding of function mammalian BAT. Herein, comprehensive analysis interscapular BAT from C57BL/6J female mice was conducted by 2DLC and high-resolution mass spectrometry to construct dataset proteins. A total 4949 nonredundant proteins were identified, 4495 quantified using iBAQ method. According values, divided into high-, middle- low-abundance The functions high-abundance mainly related glucose fatty acid oxidation produce heat for thermoregulation, while protein synthesis apoptosis, respectively. Additionally, 497 predicted have signal peptides SignalP4 software, 75 confirmed in previous studies. This study, first time, comprehensively profiled functionally annotated proteome. study will be helpful future studies focused on biomarker identification molecular mechanisms.
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