DNA-based construction at the nanoscale: emerging trends and applications.

摘要: The field of structural DNA nanotechnology has evolved remarkably-from the creation artificial immobile junctions to recent DNA-protein hybrid nanoscale shapes-in a span about 35 years. It is now possible create complex DNA-based shapes and large hierarchical assemblies with greater stability predictability, thanks development computational tools advances in experimental techniques. Although it started original goal DNA-assisted structure determination difficult-to-crystallize molecules, found its applications myriad fields. In this review, we cover some basic emerging assembly principles: hybridization, base stacking/shape complementarity, protein-mediated formation structures. We also review various nanostructures, special emphasis on biophysical that have been reported outlook, discuss further improvements such structures, explore future involving super-resolved fluorescence, single-particle cryo-electron (cryo-EM) x-ray free electron laser (XFEL) nanoscopic imaging techniques, creating new synergistic designer materials.