Epigenetic Regulation of Sox30 Is Associated with Testis Development in Mice

摘要: DNA methylation is involved in tissue-specific and developmentally regulated gene expression. Here, we screened a novel Sox30, whose might contribute to its regulation testis development mice. Sox30 member of Sox transcription factors, considered be spermatogonial differentiation spermatogenesis. However, the precise function regulatory expression pattern remain unclear. In present study, found that highly expressed adult testes but not ovaries. begins early development, testes, it specifically increased coincidentally with until adulthood. Moreover, only germ cells, also sertoli cells. hypo-methylated testis, epididymis lung mice, which expressed. By contrast, hypermethylated ovary, heart, brain, liver, kidney, spleen, pancreas, muscle, intestine, pituitary gland, blood hippocampus absent. Importantly, decreased at CpG islands observed mouse developmental after birth, associated enhanced status maintained ovaries does express during this period. Further, following demethylation treatment using 5-aza-dC, restored GC2, TM3 TM4 cell lines. This observation convincingly confirms really contributes silencing. summary, show under control status,