Inactivation of the asymmetrical displacement current in giant axons of Loligo forbesi.

摘要: 1. Asymmetrical displacement currents ('gating currents') have been recorded in intracellularly perfused squid giant axons by averaging the associated with depolarizing and hyperpolarizing pulses. The relation between 'gating current' Na inactivation was studied investigating effect of pulse duration conditioning 2. Increasing from 0-3-1 msec to 10-20 reduced off-response 50-70% its normal size; time constant 5 at +20 mV 8 degrees C. decrease current during a stronger faster; it decayed 10-26% 1-35 msec. 3. could also be demonstrated using only charge end single or averaged pulses smaller for long durations than short. A followed small long-lasting tail inward current. 4. potential -30 +10 mV, short recovery period -70 decreased on-response current'. Its size 46-71% 61-94%, respectively, interval 1-75 2-3 reduction current, measured under similar conditions, more pronounced; less 50% value. 5. observations about on are qualitatively consistent those Bezanilla & Armstrong (1974, 1975) support view that part asymmetrical is inactivated depolarization.