Method for gene function identification of orange panonychus citri

摘要: The invention relates to a method for gene function identification of orange panonychus citri. comprises the following steps: (1) preparing target dsRNA liquid: synthesizing corresponding liquid according be inhibited; (2) leaf disc by using an and washing; (3) drying until obviously shrinks, containing prepared in step 1 centrifugal tube cover, floating on is recovered original shape does not shrink; (4) putting citri disc, incubator culturing 60 80 hours at condition that temperature 22 28 DEG C, humidity 55% 65% illumination darkness ratio 14h:10h; (5) after culture ended, collecting bodies, extracting RNA, detecting expression provided 1. disclosed can used solving problem no effective introduction present.