Molecular detection method of panonychus citri Mc Gregor response abamectin stress protein encoding gene
作者： Zhong Rui , Shen Xiaomin , Li Gang , Dou Wei , Wang Jinjun
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摘要: The invention discloses a molecular detection method of panonychus citri Mc Gregor response abamectin stress protein encoding gene. comprises the following steps: firstly, extracting total RNA (Ribonucleic Acid) treated by abamectin, and then transcribing into cDNA (complementary Deoxyribonucleic Acid); secondly, carrying out real-time quantitative on RPSA, EEF2, ATPsyn-alpha, FAH, V-ATPsyn-B, CALR, sHsp, CarE-B, Actin, CRY, Fer, Vg, ATPsyn-D, TPX, TCP-1-eta GAPDH genes using qPCR (quantitative Polymerase Chain Reaction) method, wherein upstream downstream sequences detecting primer pairs 16 are shown as SEQ ID No: 1 to 32; thirdly, analyzing results obtain expression with different concentrations at time periods.
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