Die epithelial-mesenchymale Transition in der Metastasierung des duktalen Adenokarzinoms des Pankreas

摘要: Introduction: Epithelial to mesenchymal transition (EMT) is recognized as a key process during tumor invasion and metastasis. During EMT, cells of epithelial origin undergo series changes such loss cell-cell adhesion acquisition motility. However, it has been observed that having undergone EMT do not form metastases retain the original phenotype devoid characteristics. This study investigates primary its for pancreatic ductal adenocarcinoma (PDAC). Methods: Using 4 PDAC cell lines in an orthotopic mouse model PDAC, we collected tissue from tumors lymph node metastases. mRNA miRNA expression profiles were determined by micro array both sample types relevant genes was validated quantitative RT-PCR. Staining ECAD β-Catenin performed immune histochemistry histological appearance metastatic lesions graded pathologist. Statistical analysis using SPSS 16.0 GeneSpring 7.3.1 (t-test, Bonferroni Correction). Results: Key suppressing miRNAs significantly upregulated (let-7-family (P < 0.001)) non differentially regulated (miR-200 family = 0.64)) vs. tumors. Expression connected mRNAs different between tissues (ECAD, HMGA2, ZEB1). Histologically, displayed differentiation central areas with typical distribution while showing at invasive front. Metastases presented phenotype. Discussion: Our results support thesis only are able successfully Whether these reversal regain their or whether invade bloodstream colonize distant sites remains unclear focus current research.