Method of isolating and cryopreserving human white cells from whole blood

摘要: A method of salvaging granulocytes, or white cells, from buffy coat ceifuged whole blood is provided. The sequence operations leading to the separation red platelets and plasma presented in a flow diagram bag system for cryopreservation leukocytes, with freezing cells accomplished by introduction combination hydroxyethyl starch (HES), which functions as both sedimenting agent cryoprotective agent, dimethylsulfoxide (DMSO), agent. preferred 4% HES 5% DMSO. White cell interfaced collection methods so conserve all major types.