Retinal tubulin binds macular carotenoids.
作者： Paul S. Bernstein , Edward D. Tsong , Nikita A. Balashov , Robert R. Rando
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摘要: Purpose. To investigate the biochemical mechanisms responsible for specific uptake, concentration, and stabilization of carotenoids lutein zeaxanthin in macula. Methods. Soluble extracts bovine retina mixed with radioactive were purified by hydrophobic interaction, ion exchange, gel filtration chromatography. Carotenoid-associated proteins these preparations identified through photoaffinity labeling protein microsequencing. Similar purifications on human macular tissue without addition exogenous also performed. Results. Experiments retinal demonstrated that tubulin is major soluble carotenoid-binding protein. When examined, endogenous found to copurify tubulin. Conclusions. Tubulin abundance receptor axon layer fovea, where it can serve as a locus deposition high concentrations there. The binding interaction Henle's fiber could play an important role photoprotective effects against progression age-related degeneration. association tubulin, form highly ordered linear arrays, may provide explanation dichroic phenomenon Haidinger's brushes.
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