Gravity spun polycaprolactone fibres: controlling release of a hydrophilic macromolecule (ovalbumin) and a lipophilic drug (progesterone)

摘要: A hydrophilic macromolecule (ovalbumin (OVA)) and a lipophilic drug (progesterone) were incorporated in polycaprolactone (PCL) fibres by gravity spinning using particulate dispersions co-solutions of PCL steroid, respectively. loaded with 1% (w/w) OVA powder displayed pronounced burst release phase (60% the protein load) over 2 days PBS at 37°C. The profile then tended to plateau. In contrast, nanoparticle-loaded exhibited delayed initially major increase day 14. This behaviour may be useful for sequential polypeptide growth factors which are influential specific time points wound healing process. SDS–PAGE analysis revealed that molecular weight was conserved during fibre spinning. amount progesterone from increased loading but cumulative profiles (% w/w) little affected initial (1.5 3.5% or concentration solution (12.5 20% w/v). Steroid delivery rapid due high surface area permeability resulting complete loss 24 h. Released inhibited MCF-7 breast epithelial cells culture, demonstrating retention bioactivity. Gravity shows potential producing fibre-based platforms programmed bioactive molecules utility tissue engineering delivery.