Effects of Constitutive β-Catenin Activation on Vertebral Bone Growth and Remodeling at Different Postnatal Stages in Mice

摘要: Background and Objective The Wnt/β-catenin signaling pathway is essential for controlling bone mass; however, little known about the variable effects of constitutive activation β-catenin (CA-β-catenin) on growth remodeling at different postnatal stages. The goal present study was to observe CA-β-catenin vertebral in mice In particular, special attention paid whether has detrimental these processes. Methods Catnblox(ex 3) were crossed with expressing TM-inducible Cre fusion protein, which could be activated designated time points via injection tamoxifen. stabilized by tamoxifen 3 days, 2, 4, 5, 7 months after birth, lasted one month. Radiographic imaging, micro-computed tomography, immunohistochemistry, safranin O tartrate-resistant acid phosphatase staining employed remodeling. Results CA-β-catenin both early (3 days birth) late stages (2, increased formation decreased resorption, together volume. However, when stage, linear retarded, demonstrated shorter statures. addition, newly formed mainly immature located close plate. contrast, unaffected, mature evenly distributed throughout body. Conclusions can increase volume, but differential