A transgenic, mesodermal specific, Dkk1 mouse model recapitulates a spectrum of human congenital limb reduction defects.

摘要: Congenital limb reduction defects occurring in isolation of other developmental abnormalities continue to be an important medical problem which little progress has been made. Herein we generated transgenic mice expressing Dkk1 appendicular mesodermal pattern. Prx1-Dkk1 recapitulate a full spectrum human congenital defects, without issues, and have normal life-spans. Importantly, close examination the inheritance pattern suggests that there is significant degree incomplete penetrance as progeny phenotypically positive or negative, but genotypically mice, consistently give rise both normal-appearing mice. Thus, this heterogeneous phenotype reproducible with each generation regardless parents. We further go on identify mesenchymal stem cells from limited proliferative ability, differentiation potential, may explain mechanism for observed. believe prove useful future study mechanisms underlying development defects.