A comprehensive transcript map of the mouse Gnas imprinted complex
作者： Rebecca Holmes , Christine Williamson , Jo Peters , Paul Denny , Christine Wells
DOI: 10.1101/GR.955503
关键词:
摘要: The recent publication of the FANTOM mouse transcriptome has provided a unique opportunity to study diversity transcripts arising from single gene locus. We have focused on Gnas complex, as imprinting loci themselves provide insights into transcriptional regulation. Thirteen full-length cDNAs FANTOM2 set were mapped These represented one previously described transcript and 12 putative new transcripts. Of these, eight found be differentially expressed either maternal or paternal allele. Two clones extended Nespas in 3' direction, providing evidence antisense transcription spanning 30-kb genomic region summarized six units, Nespas, Nesp, Gnasxl, F7, exon 1A, Gnas. resolution map by revealed pattern alternate splicing. In addition splicing onto 2 Gnas, each sense had an short 3'UTR independent Both spliced unspliced variants imprinted found. Whereas functional significance these is not known, availability remarkable repertoire complex
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