Measurement of Renin Activity in Human Plasma

摘要: A method is described for estimating plasma renin activity by using substrate present in plasma. This differs from other indirect assay methods (1) incubation the absence of ions thus establishing conditions zero order kinetics reaction between endogeneous and (2) use angiotensinase inhibitors di-sodium ethylenediamine tetraacetic acid (EDTA) d-isopropylfluorophosphate (DFP). Recoveries added to levels similar those occurring are 85% SD±7%. The was done at pH 5.5 which shown be optimum human reacting with substrate. By incubating samples known quantities renin, evidence obtained suggesting that factors than enzyme or total concentrations affect velocity angiotensin formation. variability rate may explained existence an inhibitor activator this system a variation type