Role of activity in the selection of new electrical synapses between adult Helisoma neurons

摘要: Abstract Our aim was to determine whether neural activity in the form of sodium-dependent action potentials plays a role formation, maintenance and specificity electrical synapses between regenerating neurons. We axotomized buccal neurons mollusc, Helisoma trivolvis, placed ganglia into organ culture absence or presence tetrodotoxin (TTX), specific sodium channel blocker. Electrical coupling measured using intracellular microelectrodes positioned within soma identified Neurite outgrowth assessed by epifluorescence microscopy after filling iontophoresis with Lucifer yellow. Previous studies found that two days axotomy transient heterologous (e.g. 4 5). Five these connections disappeared new synapse stabilized paired 5. To blocking TTX affected formation synapses, we examined 5 axotomy, five axotomy. electrophysiological recordings indicated different ganglion varied their sensitivity (i.e. 19 > 4), but spontaneous abolished all 3 2 × 10−5 M TTX. Furthermore, inhibitory effects occurred seconds superfusion persisted for at least 6 days. Inhibition could be reversed normal saline. from not appreciably altered no differences incidence resistance were detected However, symmetrically elevated conclude results does play an important breaking during neuronal regeneration mollusc trivolvis.