Proteomic Analysis of MG132-Treated Germinating Pollen Reveals Expression Signatures Associated with Proteasome Inhibition
作者： Candida Vannini , Marcella Bracale , Rita Crinelli , Valerio Marconi , Paola Campomenosi
DOI: 10.1371/JOURNAL.PONE.0108811
关键词:
摘要: Chemical inhibition of the proteasome has been previously found to effectively impair pollen germination and tube growth in vitro. However, mediators these effects at molecular level are unknown. By performing 2DE proteomic analysis, 24 differentially expressed protein spots, representing 14 unique candidate proteins, were identified kiwifruit (Actinidia deliciosa) germinated presence MG132 inhibitor. qPCR analysis revealed that 11 proteins not up-regulated mRNA level, but most likely stabilized by inhibition. These predicted function various pathways including energy lipid metabolism, cell wall synthesis, synthesis/degradation stress responses. In line with this evidence, MG132-induced changes proteome accompanied an increase ATP ROS content alteration fatty acid composition.
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paperity.org参考文章(57)
A. Moscatelli, M. Cresti, Pollen Germination and Pollen Tube Growth Springer, Dordrecht. pp. 33- 65 ,(2001) , 10.1007/978-94-017-1203-3_3
Current trends in the embryology of angiosperms. Current trends in the embryology of angiosperms.. ,(2001) , 10.1007/978-94-017-1203-3
Youssef ChebliAnja Geitmann, Mechanical Principles Governing Pollen Tube Growth ,(2007)
A. Speranza, R. Crinelli, V. Scoccianti, A. Geitmann, Reactive oxygen species are involved in pollen tube initiation in kiwifruit Plant Biology. ,vol. 14, pp. 64- 76 ,(2011) , 10.1111/J.1438-8677.2011.00479.X
Ernest Beutler, Red cell metabolism ,(1971)
Ernest Beutler, Red cell metabolism: A manual of biochemical methods ,(1975)
Martin Potocký, Marek Eliáš, Bronislava Profotová, Zuzana Novotná, Olga Valentová, Viktor Žárský, Phosphatidic acid produced by phospholipase D is required for tobacco pollen tube growth. Planta. ,vol. 217, pp. 122- 130 ,(2003) , 10.1007/S00425-002-0965-4
G.L. Calzoni, A. Speranza, N. Bagni, In vitro germination of apple pollens Scientia Horticulturae. ,vol. 10, pp. 49- 55 ,(1979) , 10.1016/0304-4238(79)90068-2
Do Hee Lee, Alfred L Goldberg, Proteasome inhibitors: valuable new tools for cell biologists Trends in Cell Biology. ,vol. 8, pp. 397- 403 ,(1998) , 10.1016/S0962-8924(98)01346-4
Milena Marsoni, Marcella Bracale, Luca Espen, Bhakti Prinsi, Alfredo S. Negri, Candida Vannini, Proteomic analysis of somatic embryogenesis in Vitis vinifera. Plant Cell Reports. ,vol. 27, pp. 347- 356 ,(2008) , 10.1007/S00299-007-0438-0