Screening for novel protein targets of indomethacin in HCT116 human colon cancer cells using proteomics
作者： YAN-LI CHENG , GUI-YING ZHANG , CUI LI , JING LIN
DOI: 10.3892/OL.2013.1560
关键词:
摘要: Non-steroidal anti-inflammatory drugs, such as indomethacin (IN), inhibit colorectal cancer (CRC) growth through cyclooxygenase (COX)-independent mechanisms, however, the precise biological mechanisms are not completely understood. The aim of present study was to investigate new molecular factors potentially associated with IN in HCT116 human CRC cells, which do express COX, using a proteomic approach. total proteins from IN-treated and untreated groups were separated by immobilized pH gradient-based two-dimensional gel electrophoresis. differentially-expressed identified peptide mass fingerprint (PMF) based on matrix-assisted laser desorption/ionization time flight spectrometry. PMF maps searched SWISS-PROT/TrEMBL database PeptIdent software. Between groups, 45 differential protein spots detected 15 PMF. downregulated Wnt1-inducible signaling pathway 1, Bcl-2-related A1 mitogen-activated kinase, inhibited cell induced apoptosis. In conclusion, may exert its effects induce apoptosis suppress COX-independent pathways.
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