An Exercise to Demonstrate Soil Microbial Diversity in Introductory Environmental Science Classrooms

作者: Stephanie A. Yarwood , Elizabeth W. Sulzman

DOI: 10.2134/JNRLSE2008.37153X

关键词:

摘要: High diversity of microorganisms in the soil matrix has been focus extensive research fields biology and microbial ecology, is a key concept that students environmental or biological sciences should understand. Two activities to demonstrate highlight challenges faced studying are presented here. Using bag (soil sample) containing different candy types microbes), explore isolation molecular techniques, two strategies used diversity, asked consider limitations these methods have understanding complex communities. In second activity use this collection construct mock-phylogenetic trees. These hands-on provide with fun way learn about diversity. Student assessment scores increased by 35% after participating results from final exam questions show high percentage studentsretained major concepts demonstrated during activities.

参考文章(14)
Mary E. Stromberger, Fire vs. Metal: A Laboratory Study Demonstrating Microbial Responses to Soil Disturbances Journal of Natural Resources and Life Sciences Education. ,vol. 34, pp. 1- 7 ,(2005) , 10.2134/JNRLSE.2005.0001
Thomas P Curtis, William T Sloan, Exploring microbial diversity: A vast below Science. ,vol. 309, pp. 1331- 1333 ,(2005) , 10.1126/SCIENCE.1118176
Al Byers, Mary Ann Fitzgerald, Networking for Leadership, Inquiry, and Systemic Thinking: A New Approach to Inquiry-Based Learning Journal of Science Education and Technology. ,vol. 11, pp. 81- 91 ,(2002) , 10.1023/A:1013951531841
Achim Quaiser, Torsten Ochsenreiter, Christa Lanz, Stephan C. Schuster, Alexander H. Treusch, Jürgen Eck, Christa Schleper, Acidobacteria form a coherent but highly diverse group within the bacterial domain: evidence from environmental genomics. Molecular Microbiology. ,vol. 50, pp. 563- 575 ,(2003) , 10.1046/J.1365-2958.2003.03707.X
V Torsvik, J Goksøyr, F L Daae, High diversity in DNA of soil bacteria. Applied and Environmental Microbiology. ,vol. 56, pp. 782- 787 ,(1990) , 10.1128/AEM.56.3.782-787.1990
R. Russell Wilke, William J. Straits, Practical Advice for Teaching Inquiry-Based Science Process Skills in the Biological Sciences American Biology Teacher. ,vol. 67, pp. 534- 540 ,(2005) , 10.1662/0002-7685(2005)067[0534:PAFTIS]2.0.CO;2
Vigdis Torsvik, Lise Øvreås, Tron Frede Thingstad, Prokaryotic Diversity--Magnitude, Dynamics, and Controlling Factors Science. ,vol. 296, pp. 1064- 1066 ,(2002) , 10.1126/SCIENCE.1071698
Peter H Janssen, None, Identifying the Dominant Soil Bacterial Taxa in Libraries of 16S rRNA and 16S rRNA Genes Applied and Environmental Microbiology. ,vol. 72, pp. 1719- 1728 ,(2006) , 10.1128/AEM.72.3.1719-1728.2006
John K. Gilbert, Miriam Reiner, Thought experiments in science education : potential and current realization International Journal of Science Education. ,vol. 22, pp. 265- 283 ,(2000) , 10.1080/095006900289877
R I Amann, W Ludwig, K H Schleifer, Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiological Research. ,vol. 59, pp. 143- 169 ,(1995) , 10.1128/MR.59.1.143-169.1995