Serum Metabolic Profiling of Oocyst-Induced Toxoplasma gondii Acute and Chronic Infections in Mice Using Mass-Spectrometry.

摘要: Toxoplasma gondii is an obligate intracellular parasite causing severe diseases in immunocompromised individuals and congenitally infected neonates, such as toxoplasmosis encephalitis toxoplasmic chorioretinitis. This study aimed to determine whether serum metabolic profiling can (i) identify metabolites associated with oocyst-induced T. infection (ii) detect systemic differences between -infected mice patients controls. We performed the first global metabolomics analysis of challenged 100 sporulated Pru oocysts (Genotype II). Sera from acutely (11 days post-infection, dpi), chronically (33 dpi) control were collected analysed using LC-MS/MS platform. Following False Discovery Rate filtering, we identified 3871 2825 ions ESI + or − mode, respectively. Principal Component Analysis (PCA) Partial Least Squares Discriminant (PLS -DA) profiles that clearly differentiated -uninfected samples. Acute significantly influenced metabolonme. Our results common uniquely perturbed pathways. Acutely showed perturbations glycerophospholipid metabolism, biosynthesis amino acid, tyrosine metabolism. These findings demonstrated acute oocyst induces a perturbation metabolonme, providing new insights into mechanisms underlying changes during early stage infection.