Global Metabolomic Profiling of Mice Brains Following Experimental Infection With the Cyst-Forming Toxoplasma Gondii

摘要: The interplay between the Apicomplexan parasite Toxoplasma gondii and its host has been largely studied. However, molecular changes at metabolic level in central nervous system pathogenesis-associated metabolites during brain infection are unexplored. We used a global metabolomics strategy to identify differentially regulated affected pathways BALB/c mice with T. Pru strain 7, 14 21 days post-infection (DPI). non-targeted Liquid Chromatography-Mass Spectrometry (LC-MS) analysis detected approximately 2,755 retention time-exact mass pairs, of which more than 60 had significantly differential profiles different stages infection. These include amino acids, organic carbohydrates, fatty vitamins. biological significance these is discussed. Principal Component Analysis Orthogonal Partial Least Square-Discriminant showed metabolites’ profile change over time most significant occurring DPI. Correlated pathway imbalances were observed carbohydrate metabolism, lipid energetic metabolism acid oxidation. Eight correlated physical recovery from infection-caused illness identified. findings indicate that adopted this study sensitive approach for detecting alterations gondii-infected generated comparative tissue distinguishing infected non-infected host.