Global iTRAQ-based proteomic profiling of Toxoplasma gondii oocysts during sporulation.

摘要: Toxoplasma gondii is a medically and economically important protozoan parasite. However, the molecular mechanisms of its sporulation remain largely unknown. Here, we applied iTRAQ coupled with 2D LC–MS/MS proteomic analysis to investigate expression profile T. oocysts during sporulation. Of 2095 non-redundant proteins identified, 587 were identified as differentially expressed (DEPs). Based on Gene Ontology enrichment KEGG pathway analyses majority these DEPs found related metabolism amino acids, carbon energy. Protein interaction network generated by STRING identifiedATP-citrate lyase (ACL), GMP synthase, IMP dehydrogenase (IMPDH), poly (ADP-ribose) glycohydrolase (PARG), bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS) top five hubs. We also 25 parasite virulence factors that at relatively high levels in sporulated compared non-sporulated oocysts, which might contribute infectivity mature oocysts. Considering importance dissemination toxoplasmosis findings may help search protein targets key role infectiousness ecological success creating new opportunities for development better means disease prevention. Biological significance: The newpreventative interventions against infection relies an improved understanding proteome chemical pathways this To identify required environmentally resistant infective (immature) (mature, infective) 2DLC-MS/MS revealed changes distinguish from Many involved metabolic upregulated This work provides first quantitative characterization variations occur oocyst stage