Induction of limited DNA damage by the antitumor agent Cain's acridine.

摘要: Abstract The antitumor drug methanesulfonyl-m-anisidine, 4′-(9-acridinylamino) monohydrochloride (AMSA) (Cain9s acridine, NSC 141549), causes a limited, partially reversible decrease in size of the DNA mouse L1210 leukemia cells as analyzed by centrifugation cell lysates on alkaline sucrose gradients. Exposure for 30 min to AMSA, 2.5 µg/ml, tissue culture or 150 µg/mouse vivo results shift long-term prelabeled from >170S fractions broad band about 30S. Higher doses longer exposure times do not produce much smaller than Labeled AMSA cosediments with heavy neutral gradients which no degradation is observed, but dissociated both and 30S appears only at top gradient. On after detected when are previously lysed an medium they 70% formamide solution lysate heated 83° before centrifugation. Treatment other intercalating agents (ethidium bromide, acridine orange, phosphine orange) caused similar interpreted indicate that alkali-sensitive lesions limited number sites; this effect correlates action work showing damage chromosome structure.