Eicosanoid forming enzyme mRNA in human tissues. Analysis by quantitative polymerase chain reaction.
作者： C.D. Funk , G.A. FitzGerald
DOI: 10.1016/S0021-9258(18)98928-3
关键词:
摘要: The key enzymes in the formation of eicosanoids, including leukocyte 5-lipoxygenase (5LX), platelet 12-lipoxygenase (12LX), reticulocyte 15-lipoxygenase (15LX), prostaglandin G/H synthase cyclooxygenase, and leukotriene A4 (LTA) hydrolase have been studied extensively recent years. Little is known, however, about regulation these at gene level. We developed a quantitative polymerase chain reaction (PCR) assay to quantify mRNAs for five enzymes, as well cytoplasmic beta-actin (bACT) mRNA. Human erythroleukemia (HEL) cells, which display megakaryocytic/erythroid characteristics, were selected source RNA characterize assay. These cells expressed mRNA bACT, LTA, 12LX (in decreasing order). 5LX 15LX was undetectable. Bronchoalveolar lavage fluid obtained from asthmatic patients, primarily alveolar macrophages, contained 5LX, Treatment HEL with phorbol 12-myristate 13-acetate or steroid administration patients apparently selectively regulated certain target genes. utility this quantifying various genes blood platelets chronic myelogenous leukemia, has also demonstrated. Studies on involved biosynthetic pathways, especially when only small tissue samples are available, will be facilitated approach.
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