Functional erythropoietin receptor of the cells with neural characteristics. Comparison with receptor properties of erythroid cells.

摘要: Radioiodinated erythropoietin (Epo) was bound specifically to the cells of two non-erythroid clonal lines, PC12 and SN6, which expressed neuronal characteristics. The binding time-, cell number-, dose-dependent reversible. Although cloned Epo receptor from (derived rat adrenal medulla) identical that erythroid cells, significant differences in ligand properties between lineages were found; 1) had a single class sites with very low affinity (Kd = 16 nM), whereas classes different affinities 95 pM for high 1.9 nM sites), 2) cross-linking experiments revealed one cross-linked product 105 kDa products 140 120 cells. Taken together additional results, presence putative accessory protein(s) may alter through interaction is discussed. caused rapid increase cytosolic concentration free calcium. EGTA no effect on but completely inhibited calcium increase, indicating stimulated influx outside addition culture media elevated intracellular concentrations monoamines.