Surface enhanced Raman spectroscopy of Aurora kinases: direct, ultrasensitive detection of autophosphorylation
作者： Soumik Siddhanta , Dhanasekaran Karthigeyan , Partha P Kundu , Tapas K Kundu , Chandrabhas Narayana
DOI: 10.1039/C3RA22676J
关键词:
摘要: Highly sensitive surface enhanced Raman spectroscopy (SERS) was used to study two of the tumorigenic aurora family kinases, Aurora A and B in sub-picomole quantities. Significantly, proteins on conjugating SERS-active citrate-capped silver nanoparticles retain their enzyme activity as demonstrated by kinase assays, making SERS suitable for studies enzymatic processes. The ability differentiate between structurally similar homologous further corroborates sensitivity this technique. Based available structural information we demonstrate here that could be direct ultra-sensitive detection autophosphorylation. overall reduction intensity presence bands at 952 ∼1076 cm−1 confirmed phosphorylated state A. Thus, autophosphorylation a full length protein is first time using SERS, which critical attain its active conformation.
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