Label-Free Homogeneous Electrochemical Sensing Platform for Protein Kinase Assay Based on Carboxypeptidase Y-Assisted Peptide Cleavage and Vertically Ordered Mesoporous Silica Films
作者： Jinquan Liu , Hong Cheng , Dinggeng He , Xiaoxiao He , Kemin Wang
DOI: 10.1021/ACS.ANALCHEM.7B01739
关键词: Chemistry 、 Nanopore 、 Mesoporous silica 、 Combinatorial chemistry 、 Organic chemistry 、 Phosphorylated Peptide 、 Substrate (chemistry) 、 Peptide 、 Silicon dioxide 、 Electrochemistry 、 Cleavage (embryo)
摘要: Presented herein is a simple, robust, and label-free homogeneous electrochemical sensing platform constructed for the detection of protein kinase activity and inhibition by integration of carboxypeptidase Y (CPY)-assisted peptide cleavage reaction and vertically ordered mesoporous silica films (MSFs). In this sensing platform, the substrate peptide composed of kinase-specific recognized sequence and multiple positively charged arginine (R) residues was ingeniously designed. In the presence of protein kinase, the substrate peptide was …
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