TiO2/MWNTs nanocomposites-based electrochemical strategy for label-free assay of casein kinase II activity and inhibition
作者： Xiaoxiao He , Zhifeng Chen , Yonghong Wang , Kemin Wang , Jing Su
DOI: 10.1016/J.BIOS.2012.02.032
关键词: Biochemistry 、 Detection limit 、 Redox 、 Electrochemistry 、 Nuclear chemistry 、 Nanocomposite 、 Peptide 、 Casein kinase 2 、 Electrode 、 Phosphate 、 Chemistry
摘要: Abstract In this paper, a novel label-free electrochemical strategy has been developed for assay of casein kinase II (CK2) activity and inhibition using TiO2/MWNTs nanocomposites. This detection system takes advantage specific binding the phosphate groups with TiO2 nanoparticles fast electron transfer rate MWNTs. strategy, synthesized nanocomposite was firstly deposited on surface glassy carbon electrode (GCE). The presence MWNTs not only increased area but also promoted electron-transfer reaction. CK2, reaction resulted in phosphorylation peptide substrates. phosphorylated peptides were subsequently captured to GCE modified through nanoparticles. Then access redox probe [Fe(CN)6]3−/4− blocked. As result, decrease peak currents related concentrations providing sensing mechanism monitoring phosphorylation. can be employed CK2 low limit 0.07 U/mL. linear range 0–0.5 U/mL. Furthermore, interferences experiments PKA have studied by strategy.
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