Detection of casein kinase II by aggregation-induced emission.
作者： Zhenzhu Luan , Li Zhao , Chao Liu , Weiling Song , Peng He
DOI: 10.1016/J.TALANTA.2019.04.036
关键词:
摘要: Abstract A novel aggregation-induced emission (AIE) probe comprised of a hydrophilic protein kinase specific peptide and hydrophobic tetraphenylethene (TPE) unit was synthesized through click reaction. The prepared TPE-peptide could be completely degraded by carboxypeptidase Y (CPY) to release TPE part, which aggregated in buffer solution showed strong emission. In the presence casein (CKII), phosphorylation prevented complete degradation CPY producing nonemissive probe. Thus, developed can used detect CKII homogeneously conveniently. This detection process finished within 1.5 h with high sensitivity (0.05 mU/μL) good selectivity. method also screen inhibitor even complex biological system.
sci-hub.se 参考文章(32)
Huamin Wang, Alan Davis, Shihui Yu, Khalil Ahmed, Response of cancer cells to molecular interruption of the CK2 signal. Molecular and Cellular Biochemistry. ,vol. 227, pp. 167- 174 ,(2001) , 10.1023/A:1013112908734
Wei Song, Ru-Ping Liang, Ying Wang, Li Zhang, Jian-Ding Qiu, Green synthesis of peptide-templated gold nanoclusters as novel fluorescence probes for detecting protein kinase activity Chemical Communications. ,vol. 51, pp. 10006- 10009 ,(2015) , 10.1039/C5CC02280K
Tony Hunter, Protein kinases and phosphatases: The Yin and Yang of protein phosphorylation and signaling Cell. ,vol. 80, pp. 225- 236 ,(1995) , 10.1016/0092-8674(95)90405-0
Wei Song, Ying Wang, Ru-Ping Liang, Li Zhang, Jian-Ding Qiu, Label-free fluorescence assay for protein kinase based on peptide biomineralized gold nanoclusters as signal sensing probe. Biosensors and Bioelectronics. ,vol. 64, pp. 234- 240 ,(2015) , 10.1016/J.BIOS.2014.08.082
Andrea L. Bauman, John D. Scott, Kinase- and phosphatase-anchoring proteins: harnessing the dynamic duo Nature Cell Biology. ,vol. 4, ,(2002) , 10.1038/NCB0802-E203
Hui Tong, Yuning Hong, Yongqiang Dong, Matthias Häußler, Jacky W. Y. Lam, Zhen Li, Zufeng Guo, Zhihong Guo, Ben Zhong Tang, Fluorescent "light-up" bioprobes based on tetraphenylethylene derivatives with aggregation-induced emission characteristics Chemical Communications. pp. 3705- 3707 ,(2006) , 10.1039/B608425G
Jiang Zhou, Xiahong Xu, Xin Liu, Hao Li, Zhou Nie, Meng Qing, Yan Huang, Shouzhuo Yao, A gold nanoparticles colorimetric assay for label-free detection of protein kinase activity based on phosphorylation protection against exopeptidase cleavage Biosensors and Bioelectronics. ,vol. 53, pp. 295- 300 ,(2014) , 10.1016/J.BIOS.2013.09.070
Soumik Siddhanta, Dhanasekaran Karthigeyan, Partha P Kundu, Tapas K Kundu, Chandrabhas Narayana, Surface enhanced Raman spectroscopy of Aurora kinases: direct, ultrasensitive detection of autophosphorylation RSC Advances. ,vol. 3, pp. 4221- 4230 ,(2013) , 10.1039/C3RA22676J
Xiahong Xu, Jiang Zhou, Xin Liu, Zhou Nie, Meng Qing, Manli Guo, Shouzhuo Yao, Aptameric Peptide for One-Step Detection of Protein Kinase Analytical Chemistry. ,vol. 84, pp. 4746- 4753 ,(2012) , 10.1021/AC3001918
Jingdong Luo, Zhiliang Xie, Jacky W. Y. Lam, Lin Cheng, Ben Zhong Tang, Haiying Chen, Chengfeng Qiu, Hoi Sing Kwok, Xiaowei Zhan, Yunqi Liu, Daoben Zhu, Aggregation-induced emission of 1-methyl-1,2,3,4,5-pentaphenylsilole. Chemical Communications. pp. 1740- 1741 ,(2001) , 10.1039/B105159H