Assembly of the Murine Leukemia Virus Is Directed towards Sites of Cell-Cell Contact

摘要: We have investigated the underlying mechanism by which direct cell–cell contact enhances efficiency of cell-to-cell transmission retroviruses. Applying 4D imaging to a model retrovirus, murine leukemia virus, we directly monitor and quantify sequential assembly, release, events for individual viral particles as they happen in living cells. demonstrate that de novo assembly is highly polarized towards zones contact. Viruses assembled approximately 10-fold more frequently at cell with no change kinetics. Gag proteins were drawn adhesive formed Env glycoprotein its cognate receptor promote virus This process was dependent on cytoplasmic tail Env. lacking while still allowing formation, failed sites. Our data describe novel role establishing adhesion polarization prior becoming fusion protein allow entry into