Characteristics of the Human Lymphocyte Insulin Receptor

摘要: Abstract Insulin interactions with human lymphocytes in established cultures and isolated peripheral have been studied using 125I-insulin. Receptors circulating are indistinguishable from those found cultured cells, the lymphocyte receptors show striking similarities to structures described fat cell liver preparations. In lymphocytes, ability of insulin or analogues inhibit binding 125I-insulin is directly proportional that preparation labeled hormone purified membranes stimulate glucose oxidation cell. Binding a rapid reversible process. maximal at 15°. Dissociation rate studies revealed biphasic curve constants obtained were 8.3 x 10-5 s-1 10-4 s-1. The apparent affinity two orders sites 1.2 1010 m-1 1.1 109 m-1, whereas normal values 2.0 1.4 108 m-1. At 30° there very little degradation by 15° no detectable cells. unaffected Ca++, Mg++, EDTA. optimum pH for occurs about 7.8. cells digestion DNase, RNase, neuraminidase. However, tryptic destroys capacity bind insulin.