Genetic diversity, virulence genes and antimicrobial resistance of Salmonella Enteritidis isolated from food and humans over a 24-year period in Brazil

摘要: Salmonellosis is a major health problem worldwide. Serovar Enteritidis has been primary cause of Salmonella outbreaks in many countries. In Brazil, few molecular typing studies have performed. The aims this study were to molecularly type strains isolated Brazil order determine the genetic relationship between food and human origin, as well as, assess their pathogenic potential antimicrobial resistance. A total 128 S. from feces (67) (61) 1986 2010 studied. genotypic diversity was assessed by ERIC-PCR PFGE using XbaI, resistance disc-diffusion assay presence SPI-1, SPI-2 pSTV virulence genes PCR. results revealed that 112 exhibited similarity >85.4% 96 >80.0%. Almost all (97.6%) harbored 13 investigated. Thirty-six (28.12%) resistant nalidixic acid. conclusion, acid observed after 1996 indicative an increase use drug. It may be suggested these might descended common ancestor differed little over 24 years both contaminating humans causing disease for more than two decades Brazil.