Expression of functional KISS1 and KISS1R system is altered in human pituitary adenomas: evidence for apoptotic action of kisspeptin-10

摘要: Context: KISS1 was originally identified as a metastasis-suppressor gene able to inhibit tumor progression. products, the kisspeptins, bind G-protein-coupled receptor (KISS1R, formerly GPR54), which is highly expressed in placenta, pituitary, and pancreas, whereas mRNA mainly hypothalamus, striatum, pituitary. Objective design: KISS1/KISS1R pituitary expression profile, coupled their anti-tumoral capacities, led us hypothesize that this system may be involved biology of tumors. To explore notion, levels KISS1R were evaluated normal adenomatous pituitaries. Additionally, functionality assessed by treating dispersed adenoma cells primary culture with kisspeptin-10 evaluating intracellular calcium kinetics apoptotic rate. Results: Both simultaneous frequently lost tumors, where diverse patterns observed differed among distinct types adenomas. Measurement revealed elicits remarkable increase [Ca 2C ]i individual from four out five GH-producing adenomas studied, derived non-functioning (NFPA, nZ45) did not respond. In contrast, treatment increased rate both NFPA. Conclusions: These results provide evidence can differentially subtypes, exert functional, proapoptotic actions, thereby offer novel insights investigate therapeutic options treat these