Hoechst 33342 Induces Apoptosis in HL-60 Cells and Inhibits Topoisomerase I In Vivo
作者： Xinbo Zhang , Jenn Chen , Bruce Davis , Frederick Kiechle , None
DOI: 10.5858/1999-123-0921-HIAIHC
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摘要: Abstract Context.—Bisbenzimides (Hoechst 33342 and Hoechst 33258) are cell-permeable, adenine-thymine–specific dyes that bind to the minor groove of DNA stain DNA. induces apoptosis in BC3H-1 myocytes hepatoma cells. Objective.—To determine if or 33258 human promyelocytic leukemia cells (HL-60) inhibits topoisomerase I activity. Design.—A variety methods were used detect apoptosis: cell viability (trypan blue exclusion), nuclear fluorescence staining stained for 10 minutes), flow cytometric quantitation annexin binding phosphatidylserine, fragmentation (agarose gel electrophoresis). Topoisomerase activity was determined by a plasmid unwinding assay. Setting.—A large teaching hospital research laboratories. Patients.—None. Intervention.—None. Main Outcome Measurements.—Apoptosis is characterized decreased viability, condensation chromatin, increased phosphatidyls...
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