Inhibition of mTOR promotes hyperthermia sensitivity in SMMC-7721 human hepatocellular carcinoma cell line.

摘要: The mammalian target of rapamycin (mTOR) is a critical mediator the phosphoinositide 3-kinase/protein kinase B/mTOR signaling pathway, and mTOR activity induced following heat shock. Thermotherapy used to treat hepatocellular carcinoma (HCC). However, role in modulating thermosensitivity HCC has yet be elucidated. In present study, antisense plasmid pEGFP-C1-mTOR was transfected into SMMC-7721 cells, expression levels were analyzed by reverse transcription-polymerase chain reaction western blot analysis. thermal responses cells also examined. results revealed that sensitive treatment, cell viability significantly inhibited hyperthermia treatment (P<0.01). mRNA protein decreased post-transfection. Cell proliferation, colony-forming ability motility all cells. Flow cytometry analysis demonstrated apoptosis increased number S phase increased, cycle arrested phase. conclusion, inhibition increasing cellular inducing arrest.