Method using derivatization HPLC-DAD method to determine small-molecule halogenated carboxylic acid in medicine

摘要: The invention discloses a method using derivatization HPLC-DAD to determine small-molecule halogenated carboxylic acid in medicine.The includes: under normal temperature, nitrobenzene hydrazine reagent derivatize the so as generate products with high absorption ultraviolet visible region; reaction liquid after feeding sample, and of sample region on basis reversed phase partition chromatography principle achieve qualitative or quantitative detection acid.Due fact that band has evident redshift effect due existence nitro electron-withdrawing group benzene ring most medicine impurities thereof are weak (300-450 nanometers), simple universal pre-column is built.Methodology validation shows good specificity.