A redox factor protein, ref1, is involved in negative gene regulation by extracellular calcium.
作者： T Okazaki , U Chung , T Nishishita , S Ebisu , S Usuda
DOI: 10.1016/S0021-9258(18)46865-2
关键词:
摘要: A rise in extracellular calcium (Ca2+e) suppresses not only secretion of parathyroid hormone (PTH) but also expression the PTH gene to ensure constant plasma Ca2+ level. nuclear protein(s) a wide variety cells bound specific DNA elements (negative responsive elements, nCaREs) human sequence-specific and Ca2+e concentration-dependent manners. Our Southwestern cloning revealed that redox factor protein (ref1), which was known activate several transcription factors via alterations their state, belonged an nCaRE binding protein. The level ref1 mRNA as well its elevated by increase concentration. In gel shift assay, anti-ref1 antibody eliminated formation nCaRE-protein complex. We found there another interacting with nCaREs ref1. Further, experiments antisense-ref cDNA vector introduced into cultured suggested (nCaRE)-ref1 interaction led Ca2+e-mediated transcriptional suppression. Thus, it is concluded possesses repressor activity addition function auxiliary
sci-hub.st 参考文章(44)
Peter K. Vogt, Timothy J. Bos, jun: oncogene and transcription factor. Advances in Cancer Research. ,vol. 55, pp. 1- 35 ,(1990) , 10.1016/S0065-230X(08)60466-2
T. Okazaki, J.D. Zajac, T. Igarashi, E. Ogata, H.M. Kronenberg, Negative regulatory elements in the human parathyroid hormone gene. Journal of Biological Chemistry. ,vol. 266, pp. 21903- 21910 ,(1991) , 10.1016/S0021-9258(18)54722-0
T Okazaki, T Igarashi, H M Kronenberg, 5'-flanking region of the parathyroid hormone gene mediates negative regulation by 1,25-(OH)2 vitamin D3. Journal of Biological Chemistry. ,vol. 263, pp. 2203- 2208 ,(1988) , 10.1016/S0021-9258(18)69191-4
L J Walker, C N Robson, E Black, D Gillespie, I D Hickson, Identification of residues in the human DNA repair enzyme HAP1 (Ref-1) that are essential for redox regulation of Jun DNA binding. Molecular and Cellular Biology. ,vol. 13, pp. 5370- 5376 ,(1993) , 10.1128/MCB.13.9.5370
E. M. Brown, Extracellular Ca2+ sensing, regulation of parathyroid cell function, and role of Ca2+ and other ions as extracellular (first) messengers Physiological Reviews. ,vol. 71, pp. 371- 411 ,(1991) , 10.1152/PHYSREV.1991.71.2.371
S. Xanthoudakis, G. Miao, F. Wang, Y.C. Pan, T. Curran, Redox activation of Fos‐Jun DNA binding activity is mediated by a DNA repair enzyme. The EMBO Journal. ,vol. 11, pp. 3323- 3335 ,(1992) , 10.1002/J.1460-2075.1992.TB05411.X
Walter Schaffner, How do different transcription factors binding the same DNA sequence sort out their jobs? Trends in Genetics. ,vol. 5, pp. 37- 39 ,(1989) , 10.1016/0168-9525(89)90017-6
Y Nishizuka, Studies and perspectives of protein kinase C Science. ,vol. 233, pp. 305- 312 ,(1986) , 10.1126/SCIENCE.3014651
M. S. Kapiloff, J. M. Mathis, C. A. Nelson, C. R. Lin, M. G. Rosenfeld, Calcium/calmodulin-dependent protein kinase mediates a pathway for transcriptional regulation. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 3710- 3714 ,(1991) , 10.1073/PNAS.88.9.3710
H Bading, D. Ginty, M. Greenberg, Regulation of gene expression in hippocampal neurons by distinct calcium signaling pathways Science. ,vol. 260, pp. 181- 186 ,(1993) , 10.1126/SCIENCE.8097060