Genomic Insights of Halophilic Planococcus maritimus SAMP MCC 3013 and Detail Investigation of Its Biosurfactant Production.

摘要: Moderate halophilic bacteria thrive in saline conditions and produce biosurfactant (BS) which facilitates the oil scavenging activity polluted surroundings. Production of such unusual bioactive molecules plays a vital role for their survival an extreme adverse environment. Current research deals with isolation Planococcus maritimus strain SAMP MCC 3013 from Indian Arabian coastline sea water BS production. The bacterium tolerated up to 2.7 M NaCl demonstrating osmotic stress bearable physiological systems. We used integrated approach explore genomic insight displayed presence gene biosynthesis. genome analysis revealed this potential be intrinsic strain. Preliminary screening techniques viz., surface tension (SFT), drop collapse (DC) displacement (OD) showed as potent producer. reduced SFT phosphate buffer (PBS) pH: 7.0 72 30 mN/m critical micelle concentration (CMC) value 1.3 mg/mL. Subsequent investigation on chemical characterization, using thin layer chromatography (TLC), Fourier transform infrared spectroscopy (FT-IR), Nuclear magnetic resonance (1H NMR 13C NMR), Liquid Chromatography Mass Spectrometry (LC-MS) terpene containing having sugar, lipid moieties. sequence P. complete genes pathway synthesis terpenoid. Probably terpenoid is accountable backbone molecule production, but later stages conversion could not found. Moreover, it important highlight that till today; no single report documents in-detailed physico-chemical characterization sp. Based functional properties, term denoted surfactant produced by maritimus.