Characterization of pancreatic transcription factor Pdx-1 binding sites using promoter microarray and serial analysis of chromatin occupancy

摘要: The homeobox transcription factor Pdx-1 is necessary for pancreas organogenesis and beta cell function, however, most Pdx-1-regulated genes are unknown. To further the understanding of in biology, we have characterized its genomic targets NIT-1 cells, a mouse insulinoma line. identify novel targets, developed microarray that includes traditional promoters as well non-coding conserved elements, micro-RNAs, elements identified through an unbiased approach termed serial analysis chromatin occupancy. In total, 583 new target were identified, many which contribute to energy sensing insulin release pancreatic cells. By analyzing 31 protein-coding genes, show 29 expressed cells and, these, 68% down- or up-regulated expressing dominant negative mutant Pdx-1. We additionally also interact with NeuroD1/BETA2, including micro-RNA miR-375, known regulator secretion.