A multiplex real-time PCR for quantification of HIV-1 DNA and the human albumin gene in CD4+ cells.
作者： LARS E. ERIKSSON , THOMAS LEITNER , BRITTA WAHREN , ANN-CHARLOTTE BOSTROM , KERSTIN I. FALK
DOI: 10.1034/J.1600-0463.2003.1110605.X
关键词:
摘要: We have established a simple system for measuring HIV-1 DNA load in CD4+ cells. In multiplex configuration, conserved region the pol gene and section of human albumin were simultaneously amplified to estimate number copies per cellular genome. An Epstein-Barr virus (EBV) standard was used calibrate quantification. Our PCR tested on different vitro developed strains longitudinal samples from eight patients. The able amplify both vivo various genetic compositions. all patients, detected ranged between 0.17 51x10-3 cell could be monitored longitudinally, including long-term PI-ART STI. measured may select best time institution or re-institution therapy.
sci-hub.se 参考文章(37)
Avidan U Neumann, Roland Tubiana, Vincent Calvez, Catherine Robert, Tai-Sheng Li, Henri Agut, Brigitte Autran, Christine Katlama, Comet Study Group, HIV-1 rebound during interruption of highly active antiretroviral therapy has no deleterious effect on reinitiated treatment AIDS. ,vol. 13, pp. 677- 683 ,(1999) , 10.1097/00002030-199904160-00008
Kunal Saha, Jianchao Zhang, Anil Gupta, Rajnish Dave, Meron Yimen, Bouchra Zerhouni, Isolation of primary HIV-1 that target CD8+ T lymphocytes using CD8 as a receptor. Nature Medicine. ,vol. 7, pp. 65- 72 ,(2001) , 10.1038/83365
Richard D Hockett, Michael S Saag, J.Michael Kilby, Greg Sfakianos, Theresa B Wakefield, R.Pat Bucy, Stability in the HIV vDNA pool in peripheral CD4+ T cells of untreated patients by single tube quantitative PCR Journal of Virological Methods. ,vol. 87, pp. 1- 12 ,(2000) , 10.1016/S0166-0934(00)00139-7
Sharon Safrin, Carl Grunfeld, Fat distribution and metabolic changes in patients with HIV infection AIDS. ,vol. 13, pp. 2493- 2505 ,(1999) , 10.1097/00002030-199912240-00002
Lars E. Eriksson, Gun Nordstrom, Torsten Berglund, Eric Sandstrom, The health related quality of life in a Swedish sample of HIV-infected persons Journal of Advanced Nursing. ,vol. 32, pp. 1213- 1223 ,(2000) , 10.1046/J.1365-2648.2000.01592.X
M Popovic, M. Sarngadharan, E Read, R. Gallo, Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS Science. ,vol. 224, pp. 497- 500 ,(1984) , 10.1126/SCIENCE.6200935
Elisabetta Riva, Mauro Pistello, Pasquale Narciso, Gianpiero D'Offizi, Patrizia Isola, Vincenzo Galati, Ombretta Turriziani, Valerio Tozzi, Laura Vincenzi, Ferdinando Dianzani, Guido Antonelli, Decay of HIV Type 1 DNA and Development of Drug-Resistant Mutants in Patients with Primary HIV Type 1 Infection Receiving Highly Active Antiretroviral Therapy AIDS Research and Human Retroviruses. ,vol. 17, pp. 1599- 1604 ,(2001) , 10.1089/088922201753342004
Jose Catalan, Psychiatric manifestations of HIV disease Baillière's Clinical Gastroenterology. ,vol. 4, pp. 547- 562 ,(1990) , 10.1016/0950-3528(90)90016-A
Daniel C. Douek, Jason M. Brenchley, Michael R. Betts, David R. Ambrozak, Brenna J. Hill, Yukari Okamoto, Joseph P. Casazza, Janaki Kuruppu, Kevin Kunstman, Steven Wolinsky, Zvi Grossman, Mark Dybul, Annette Oxenius, David A. Price, Mark Connors, Richard A. Koup, HIV preferentially infects HIV-specific CD4+ T cells Nature. ,vol. 417, pp. 95- 98 ,(2002) , 10.1038/417095A
Kristina Koppel, Göran Bratt, Mats Eriksson, Eric Sandström, Serum lipid levels associated with increased risk for cardiovascular disease is associated with highly active antiretroviral therapy (HAART) in HIV-1 infection International Journal of Std & Aids. ,vol. 11, pp. 451- 455 ,(2000) , 10.1258/0956462001916236