Proteomics and antivenomics of Papuan black snake (Pseudechis papuanus) venom with analysis of its toxicological profile and the preclinical efficacy of Australian antivenoms.

摘要: Abstract The Papuan black snake ( Pseudechis papuanus Serpentes: Elapidae) is endemic to Papua New Guinea, Indonesian and Australia's Torres Strait Islands. We have investigated the biological activity proteomic composition of its venom. P . venom proteome dominated by a variety (n ≥ 18) PLA 2 s, which together account for ~ 90% proteins, set low relative abundance including short-neurotoxic 3FTx (3.1%), 3–4 PIII-SVMPs (2.8%), 3 cysteine-rich secretory proteins (CRISP; 2.3%) 1–3 l -amino acid oxidase (LAAO) molecules (1.6%). Probing cDNA library with specific primers resulted in elucidation full-length nucleotide sequences six new toxins, vespryn NGF not found proteome, calglandulin protein involved toxin expression glands. Intravenous injection mice induced lethality, intravascular haemolysis, pulmonary congestion oedema, anticoagulation after intravenous injection, these effects are mainly due action s. This study also evaluated vivo preclinical efficacy Australian polyvalent Seqirus antivenoms. These antivenoms were effective neutralising lethal, anticoagulant activities mice. On other hand, all tested using an antivenomic approach exhibited strong immunorecognition components. results suggest that 1 may provide paraspecific protection against humans. Significance paragraph toxicological profile snake, , large diurnal southern coast Guinea handful close offshore islands, investigated. hemolysis, edema, anticoagulation, death. could be assigned molecules, dominate proteome. showed or support continued recommendation clinical management envenoming Australia, Province.