Girdin: an essential component of pre-replicative complex in human cells

摘要: Abstract A central event in the initiation of DNA replication eukaryotes is assembly pre-replicative complex (pre-RC) on specific chromatin sites known as origins. The pre-RC process differs between budding and fission yeasts. In yeast, Sap1 directly participates assembly, together with four factors: ORC, Cdc18/Cdc6, Cdt1, MCM. metazoans, nature origins not defined mechanism remains incompletely known. this study, Girdin was identified an essential factor human cells. Similar to activity Sap1, binds origins, interacts required for due its role recruitment Cdc6 Thus, or metazoans are including two elements, one bound by ORC other Girdin.