Method for preparing HPV (human papillomavirus) antigen specific CTL (cytotoxic T lymphocyte)

摘要: The invention discloses a method for preparing HPV (human papillomavirus) antigen specific CTL (cytotoxic T lymphocyte) and particularly preparation of HLA-A2402 restrictive anti-HPV CTL. According to the method, peripheral blood mononuclear cell is collected through single collection or venous collection, presentation function B cells enhanced with CpG ODN 2395, supporting peptide are used stimulating cell, rhIL-2, rhIL-7, rhIL-15 rhIL-21 jointly promote growth cell. target prepared has characteristics that simple, cycle short, cost low, multiplication capacity killing activity high, viability high like, can be immunotherapy infection related diseases including cervical cancer.