Mitochondrial CK (EC 2.7.3.2) in the human heart

摘要: CKm, a mitochondrial form of CK, can be isolated from human cardiac muscle. It occurs in two forms, separable on the basis difference net charge. Both molecules have dimeric structure and consist identical subunits. Upon incubation serum, CK isozyme with faster cathodal mobility is rapidly converted into slower form, which has an isoelectric point 6.94. This makes it impossible to separate this slow CKM MM (isoelectric point: 6.86) by means any method that separates differences Furthermore almost molecular weight. The only between we been able detect different behaviour against anti-M antibodies.