Spatial regulation of specific gene expression through photoactivation of RNAi

作者: Tamaki Endoh , Masahiko Sisido , Takashi Ohtsuki

DOI: 10.1016/J.JCONREL.2009.04.015

关键词:

摘要: In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and light source to trigger RNAi. We demonstrate photo-dependent gene silencing several dyes different excitation wavelengths. Additionally, use from halogen lamp photomask produce photopatterned RNAi, laser single-cell RNAi on cell culture plates.

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